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Optimisation of antioxidant hydrolysate production from sweet potato protein and effect of in vitro gastrointestinal digestion

Author:
Zhang, Miao, Mu, Tai‐Hua
Source:
International journal of food science & technology 2016 v.51 no.8 pp. 1844-1850
ISSN:
0950-5423
Subject:
antioxidant activity, dietary supplements, free radical scavengers, hydrolysates, in vitro digestion, molecular weight, pH, potato protein, response surface methodology, sweet potatoes, temperature
Abstract:
In this study, response surface methodology (RSM) was applied to optimise antioxidant hydrolysate production from sweet potato protein (SPP). The effects of enzyme‐to‐substrate ratio, pH value and reaction temperature on ·OH radical scavenging activity and Fe²⁺‐chelating activity of antioxidant hydrolysates from SPP were investigated. The maximum ·OH radical scavenging activity (40.10%) and Fe²⁺‐chelating activity (74.37%) of SPP hydrolysates (SPPH) were obtained at an enzyme‐to‐substrate ratio of 4%, pH value of 7.8 and reaction temperature of 57 °C, which was in agreement with the predicted value (40.11% and 74.83%, respectively) estimated by RSM. The simulated in vitro gastrointestinal digestion (GID) dramatically modified molecular weights distribution, increased peptide (<3 kDa) concentration and highly retained antioxidant activity of SPPH, indicating potentially utilisation of SPPH as a functional supplement in food system.
Agid:
5373506