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Fabrication of Boehmite/Alginate Hybrid Beads for Efficient Enzyme Immobilization

Ai, Qinghong, Yang, Dong, Zhu, Yuanyuan, Jiang, Zhongyi
Industrial & Engineering Chemistry Research 2013 v.52 no.42 pp. 14898-14905
alcohol dehydrogenase, boehmite, calcium alginate, carbon dioxide, chemistry, electrostatic interactions, encapsulation, engineering, fluorescence, hybridization, immobilized enzymes, yeasts
A novel kind of matrix for enzyme immobilization, the boehmite/alginate hybrid beads, was fabricated under mild conditions in this study. The boehmite particles were first prepared via a mild sol–gel technology and were then entrapped into the inherent network of calcium alginate. The characterization results suggest that the hybridization between alginate and boehmite belongs to a physical blending, in which the electrostatic interaction acts an important role. Yeast alcohol dehydrogenase (YADH), which is of high importance for the transformation of CO₂, was encapsulated in the boehmite/alginate hybrid beads. The enzyme was loaded as high as 99.4% and uniformly distributed in these hybrid beads according to the fluorescence experiment. It is worthy of noting that the encapsulated YADH can almost reach “zero leaching” after incubation for 67 h and preserve 86.6% activity after 12-recycle using. This kind of hybrid materials may become a promising matrix for the immobilization of industrial enzymes.