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A Reaction-Based Fluorescent Probe for Selective Imaging of Carbon Monoxide in Living Cells Using a Palladium-Mediated Carbonylation

Author:
Michel, Brian W., Lippert, Alexander R., Chang, Christopher J.
Source:
Journal of the American Chemical Society 2012 v.134 no.38 pp. 15668-15671
ISSN:
1520-5126
Subject:
animals, carbon monoxide, chemistry, fluorescent dyes, hydrogen sulfide, image analysis, nitric oxide, tissues
Abstract:
Carbon monoxide is a member of the gasotransmitter family, which also includes NO and H₂S, and has been implicated in a variety of pathological and physiological conditions. Whereas exogenous therapeutic additions of CO to tissues and whole animals have been well-studied, the real-time spatial and temporal tracking of CO at the cellular level remains an open challenge. Here we report a new type of turn-on fluorescent probe for selective CO detection based on palladium-mediated carbonylation reactivity. CO Probe 1 (COP-1) is capable of detecting CO both in aqueous buffer and in live cells with high selectivity over a range of biologically relevant reactive small molecules, providing a potentially powerful approach for interrogating its chemistry in biological systems.
Agid:
5386661