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A Rationally Designed Pyrrolysyl-tRNA Synthetase Mutant with a Broad Substrate Spectrum

Author:
Wang, Yane-Shih, Fang, Xinqiang, Wallace, Ashley L., Wu, Bo, Liu, Wenshe R.
Source:
Journal of the American Chemical Society 2012 v.134 no.6 pp. 2950-2953
ISSN:
1520-5126
Subject:
Escherichia coli, green fluorescent protein, mutants, nonsense mutation, phenylalanine
Abstract:
Together with tRNACUAᴾʸˡ, a rationally designed pyrrolysyl-tRNA synthetase mutant N346A/C348A has been successfully used for the genetic incorporation of a variety of phenylalanine derivatives with large para substituents into superfolder green fluorescent protein at an amber mutation site in Escherichia coli. This discovery greatly expands the genetically encoded noncanonical amino acid inventory and opens the gate for the genetic incorporation of other phenylalanine derivatives using engineered pyrrolysyl-tRNA synthetase-tRNACUAᴾʸˡ pairs.
Agid:
5387605