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Extracellular protease in Actinomycetes culture supernatants inhibits and detaches Staphylococcus aureus biofilm formation
- Park, Joo-Hyeon, Lee, Jin-Hyung, Kim, Chang-Jin, Lee, Jae-Chan, Cho, Moo Hwan, Lee, Jintae
- Biotechnology letters 2012 v.34 no.4 pp. 655-661
- Staphylococcus aureus, biofilm, antibiotic resistance, proteinases, Actinomycetales, medical equipment, anti-infective agents, pathogens
- Bacterial biofilms are associated with chronic infections due to their resistance to antimicrobial agents. Staphylococcus aureus is a versatile human pathogen and can form biofilms on human tissues and diverse medical devices. To identify novel biofilm inhibitors of S. aureus, the supernatants from a library of 458 Actinomycetes strains were screened. The culture supernatants (1% v/v) of more than 10 Actinomycetes strains inhibited S. aureus biofilm formation by more than 80% without affecting the growth. The culture supernatants of these biofilm-reducing Actinomycetes strains contained a protease (equivalent to 0.1 μg proteinase K ml−1), which both inhibited S. aureus biofilm formation and detached pre-existing S. aureus biofilms. This study suggests that protease treatment could be a feasible tool to reduce and eradicate S. aureus biofilms.