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Automatic Pseudorotaxane Formation Targeting on Nucleic Acids Using a Pair of Reactive Oligodeoxynucleotides

Author:
Onizuka, Kazumitsu, Nagatsugi, Fumi, Ito, Yoshihiro, Abe, Hiroshi
Source:
Journal of the American Chemical Society 2014 v.136 no.20 pp. 7201-7204
ISSN:
1520-5126
Subject:
DNA, RNA, gels, methodology, nanotechnology, oligodeoxyribonucleotides, pH, polyacrylamide
Abstract:
Here we report a novel method to form a pseudorotaxane architecture using only a pair of reactive oligodeoxyribonucleotides (ODNs), which we designed and synthesized, and then performed the pseudorotaxane formation reaction with both DNA and RNA oligonucleotides. The reaction proceeded smoothly without any extra reagents at 37 °C and pH 7.2, leading to the formation of a stable complex on a denaturing polyacrylamide gel. Interestingly, the pseudorotaxane was formed with the cyclized ODN reversibly by the slipping process. This new pseudorotaxane formation represents a promising method for developing new DNA nanotechnologies and antisense oligonucleotides.
Agid:
5391494