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Influence of various antioxidants added to TCM-199 on post-thaw bovine sperm parameters, DNA integrity and fertilizing ability
- Sarıözkan, Serpil, Bucak, Mustafa Numan, Tuncer, Pürhan B., Büyükleblebici, Serhat, Cantürk, Fazile
- Cryobiology 2014 v.68 no.1 pp. 129-133
- DNA, DNA damage, acrosome, antioxidants, carnitine, cattle, cysteine, glutamine, methionine, pregnancy rate, protective effect, semen extenders, sperm motility
- Supplementation of the semen extender with antioxidants did not produce any significant effect on CASA and progressive motilities and sperm motility characteristics, in comparison to the control group (P>0.05).For sperm acrosome and total abnormalities, TCM-199 supplemented with cysteine (2.60±0.24% and 4.80±0.20%), glutamine (2.80±0.20% and 6.40±0.40%), carnitine (2.60±0.24% and 6.00±0.63%) and methionine (3.40±0.51% and 9.20±0.86%) at doses of 2mM provided a better protective effect, compared to that of the controls (8.00±0.44 and 15.60±1.895). As regards sperm membrane integrity, supplementation with 2mM of glutamine and methionine (56.00±1.70% and 62.40±1.78%, respectively) resulted in higher rates, when compared to the control group (41.40±4.74%). According to the results of the COMET assay, only the use of TCM-199 supplemented with 2mM of cysteine reduced DNA damage and resulted in percentages of sperm with damaged DNA (2.17±0.18%) lower than those of the control group (3.16±0.32%) (P<0.001). For pregnancy rates, there were no significant differences among the extender groups (P>0.05).