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Twinkle, twinkle little star: Photoswitchable fluorophores for super-resolution imaging
- Chozinski, Tyler J., Gagnon, Lauren A., Vaughan, Joshua C.
- FEBS letters 2014 v.588 pp. 3603-3612
- fluorescence, fluorescence microscopy, fluorescent dyes, image analysis
- Photoswitchable fluorescent probes are key elements of newly developed super-resolution fluorescence microscopy techniques that enable far-field interrogation of biological systems with a resolution of 50nm or better. In contrast to most conventional fluorescence imaging techniques, the performance achievable by most super-resolution techniques is critically impacted by the photoswitching properties of the fluorophores. Here we review photoswitchable fluorophores for super-resolution imaging with discussion of the fundamental principles involved, a focus on practical implementation with available tools, and an outlook on future directions.