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Vibrio vulnificus glycogen branching enzyme preferentially transfers very short chains: N1 domain determines the chain length transferred
- Jo, Hye-Jin, Park, Sunghoon, Jeong, Hee-Gon, Kim, Jung-Wan, Park, Jong-Tae
- FEBS letters 2015 v.589 pp. 1089-1094
- 1,4-alpha-glucan branching enzyme, Escherichia coli, Vibrio vulnificus, glycogen, mutants
- The glycogen branching enzyme from Vibrio vulnificus (VvGBE) transfers short side chains (DP 3–5) significantly greater than any other bacterial glycogen branching enzyme (GBE). To elucidate the role of the N-domain of VvGBE in the unique branching pattern, domain-truncated (N1 and N) and N1-domain-swapped (with VvGBE N1 replacing the counter part of Escherichia coli GBE) mutants were constructed. The truncation mutants synthesized branched products with a greatly reduced proportion of short chains. The swapping mutant exhibited a branching pattern of the short chain region similar to that of VvGBE. We conclude that the N1-domain of VvGBE has a crucial role in the determination of the branching pattern of glycogen.