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Epigenetic harnessing of HCV via modulating the lipid droplet-protein, TIP47, in HCV cell models
- El-Ekiaby, Nada M., Mekky, Radwa Y., El Sobky, Shereen A., Elemam, Noha M., El-Sayed, Mohammed, Esmat, Gamal, Abdelaziz, Ahmed I.
- FEBS letters 2015 v.589 pp. 2266-2273
- biopsy, droplets, epigenetics, hepatitis C, lipids, liver, microRNA, models, patients
- This study aimed at identifying potential microRNAs that modulate hepatic lipid droplets (LD) through targeting the Tail interacting protein of 47kDa (TIP47) in HCV infection.Bioinformatics analysis revealed that miR-148a and miR-30a potentially target TIP47. Expression profiling showed that both microRNAs were downregulated, while TIP47 was upregulated in liver biopsies of HCV-infected patients. Forcing the expression of both microRNAs in JFH-I infected, oleic acid-treated Huh7 cells, significantly suppressed TIP47 expression and reduced cellular LDs with marked decrease in viral RNA. This study shows that miR-148a and miR-30a, regulate TIP47 expression and LDs in HCV infected cells.