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Recognition of xyloglucan by the crystalline cellulose-binding site of a family 3a carbohydrate-binding module

Author:
Hernandez-Gomez, Mercedes C., Rydahl, Maja G., Rogowski, Artur, Morland, Carl, Cartmell, Alan, Crouch, Lucy, Labourel, Aurore, Fontes, Carlos M.G.A., Willats, William G.T., Gilbert, Harry J., Knox, J. Paul
Source:
FEBS letters 2015 v.589 no.18 pp. 2297-2303
ISSN:
0014-5793
Subject:
carbohydrate binding, cell walls, cellulose, ligands, xyloglucans
Abstract:
Type A non-catalytic carbohydrate-binding modules (CBMs), exemplified by CtCBM3acipA, are widely believed to specifically target crystalline cellulose through entropic forces. Here we have tested the hypothesis that type A CBMs can also bind to xyloglucan (XG), a soluble β-1,4-glucan containing α-1,6-xylose side chains. CtCBM3acipA bound to xyloglucan in cell walls and arrayed on solid surfaces. Xyloglucan and cellulose were shown to bind to the same planar surface on CBM3acipA. A range of type A CBMs from different families were shown to bind to xyloglucan in solution with ligand binding driven by enthalpic changes. The nature of CBM-polysaccharide interactions is discussed.
Agid:
5393328