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Low-temperature microRNA expression in the painted turtle, Chrysemys picta during freezing stress
- Biggar, Kyle K., Storey, Kenneth B.
- FEBS letters 2015 v.589 pp. 3665-3670
- Chrysemys picta, cold stress, cold tolerance, freezing, messenger RNA, microRNA, stress response, turtles, viability
- Natural freeze tolerance depends on cellular adaptations that address the multiple stresses imposed on cells during freezing. These adaptations preserve viability by suppressing energy-expensive cell processes in the frozen state. In this study, we explore the freeze-responsive expression of microRNA in hatchling painted turtles exposed to 20h freezing. Furthermore, we also explore the possibility of unique temperature-sensitive microRNA targeting programs that aid in adapting turtles for survival in the frozen state. Interestingly, two freeze-responsive ‘cryo-miRs’ (cpm-miR-16 and cpm-miR-21) were found to have unique low-temperature mRNA targets enriched in biological processes that are known to be part of the stress response.