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PCR-based prediction of type I β-exotoxin production in Bacillus thuringiensis strains
- Sauka, Diego H., Pérez, Melisa P., López, Nanci N., Onco, María I., Berretta, Marcelo F., Benintende, Graciela B.
- Journal of invertebrate pathology 2014 v.122 pp. 28-31
- Bacillus thuringiensis, Musca domestica, adenine, bioassays, high performance liquid chromatography, nucleosides, oligosaccharides, polymerase chain reaction, prediction, thermal stability, toxicity
- Some Bacillus thuringiensis strains secrete type I β-exotoxin, which is a non-specific insecticidal and thermostable adenine nucleoside oligosaccharide. Toxicity bioassays and HPLC are traditional methods for detecting β-exotoxin. With the aim of establish a first rapid approach for prediction of type I β-exotoxin production, two PCR-based methods were successfully evaluated in B. thuringiensis strains and native isolates. In order to validate a reliable technology, results obtained by this method were correlated with that obtained from Musca domestica bioassays.