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A bacteriocin-like substance produced from Lactobacillus pentosus 39 is a natural antagonist for the control of Aeromonas hydrophila and Listeria monocytogenes in fresh salmon fillets

Author:
Anacarso, Immacolata, Messi, Patrizia, Condò, Carla, Iseppi, Ramona, Bondi, Moreno, Sabia, Carla, de Niederhäusern, Simona
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2014 v.55 no.2 pp. 604-611
ISSN:
0023-6438
Subject:
Aeromonas hydrophila, Lactobacillus pentosus, Listeria monocytogenes, antagonists, bacteriocins, biopreservation, fillets, pathogens, psychrotrophic bacteria, ready-to-eat foods, refrigeration, salmon, seafoods, shelf life, temperature
Abstract:
We studied the ability of Lactobacillus pentosus 39, a BLS (Bacteriocin-like substance)-producing strain, to control the growth of Aeromonas hydrophila ATCC 14715 and Listeria monocytogenes ATCC 19117 artificially added to fresh salmon fillets at refrigeration temperatures and under simulated cold-chain break conditions.At refrigeration temperatures, Lb. pentosus 39 protective culture and its putative bacteriocin significantly reduced A. hydrophila counts compared with the control (2.1 and 1.4 log CFU/g reductions, respectively). Similar behaviour was observed for L. monocytogenes (3.6 and 1.3 log CFU/g reductions, respectively).Under simulated cold-chain break conditions, an increase in temperature (30°C for 12h) produced an evident increase in the development of A. hydrophila, L. monocytogenes, but also of Lb. pentosus 39, with a consequent increase in BLS production. This condition resulted in a greater reduction of both pathogens compared with samples stored at 4°C throughout the experiment (2.8 log CFU/g reduction for A. hydrophila, 5.8 log CFU/g reduction for L. monocytogenes). In samples treated with the putative bacteriocin alone, a less marked decrease was observed.Our study demonstrates the capability of Lb. pentosus 39 to control the growth of psychrotrophic bacteria in an experimental seafood model system. A similar biopreservation technology could provide more prolonged shelf-life during storage of ready-to-eat seafood, ensuring safety, even under extreme conditions.
Agid:
5399068