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Characterization and cytocompatibility of an antibiotic/chitosan/cyclodextrins nanocoating on titanium implants

Mattioli-Belmonte, Monica, Cometa, Stefania, Ferretti, Concetta, Iatta, Roberta, Trapani, Adriana, Ceci, Edmondo, Falconi, Mirella, De Giglio, Elvira
Carbohydrate polymers 2014 v.110 pp. 173-182
Staphylococcus aureus, X-ray photoelectron spectroscopy, adhesion, antibiotics, cell viability, chitosan, ciprofloxacin, coatings, cross infection, gamma-cyclodextrin, gene expression, high performance liquid chromatography, nanoparticles, particle size, prostheses, titanium, transmission electron microscopy, zeta potential
A novel ciprofloxacin loaded chitosan nanoparticle-based coating onto titanium substrates has been developed and characterized to obtain an orthopaedic implant surface able to in situ release the antibiotic for the prevention of post-operative infections. Ciprofloxacin loaded chitosan nanoparticles were obtained using the combination of sulfobutyl ether-beta-cyclodextrin and gamma-cyclodextrin. The resulting nanoparticulate system was characterized by TEM, HPLC and XPS. Particle size was in the range 426–552nm and zeta potential values were around +30mV. This antibacterial coating was able to in vitro inhibit two nosocomial Staphylococcus aureus strains growth, with a reduction of about 20 times compared to controls. No impairment in MG63 osteoblast-like cells viability, adhesion and gene expression were detected at 48h, 7 and 14 days of culture. Overall, the investigated coating represents a promising candidate for the development of a new antibiotic carrier for titanium implants.