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A biocompatible calcium salt of hyaluronic acid grafted with polyacrylic acid

Author:
Nakagawa, Yoshiyuki, Nakasako, Satoshi, Ohta, Seiichi, Ito, Taichi
Source:
Carbohydrate polymers 2015 v.117 pp. 43-53
ISSN:
0144-8617
Subject:
adhesion, biocompatibility, calcium, carboxylic ester hydrolases, drugs, enzymatic hydrolysis, hyaluronic acid, hyaluronoglucosaminidase, intraperitoneal injection, mice, mixing, peritoneum, polyacrylic acid, polymerization, subcutaneous injection
Abstract:
We have synthesized hyaluronic acid (HA) grafted with polyacrylic acid (PAA) via controlled radical polymerization (CRP) in aqueous media. The grafted HA (HA-g-PAA) showed slow degradation by hyaluronidase compared with unmodified HA as a result of the steric hindrance produced by grafted PAA, and PAA was detached by hydrolysis and enzymatic degradation by lipase. It formed an insoluble salt immediately after mixing with Ca2+ by the binding between grafted PAA and Ca2+. Both HA-g-PAA and its salt showed good biocompatibility, especially to mesothelial cells in vitro. Finally, they were administered into mice subcutaneously and intraperitoneally. The residue of the material was observed 7 days after subcutaneous administration, while the material was almost cleared from the peritoneum 7 days after intraperitoneal administration with or without Ca2+. HA-g-PAA is expected to be applicable to medical uses such as drug delivery in the peritoneum and for materials preventing peritoneal adhesion.
Agid:
5408455