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Chemical characterization and in vitro antitumor activity of a single-component polysaccharide from Taxus chinensis var. mairei
- Wu, Mianbin, Zhang, Feifei, Yu, Zhangping, Lin, Jianping, Yang, Lirong
- Carbohydrate polymers 2015 v.133 pp. 294-301
- Taxus wallichiana var. mairei, antineoplastic activity, apoptosis, arabinose, flow cytometry, galactose, gas chromatography, gel chromatography, glucose, glycosidic linkages, ion exchange, leaves, mannose, methylation, molecular weight, nuclear magnetic resonance spectroscopy, oxidation, polysaccharides, rhamnose, xylose
- An alkali-soluble single-component polysaccharide, named CPTC-2, was isolated and purified from the leaves of Taxus chinensis var. mairei. by ion-exchange and gel-permeation chromatography in series. The weight-average molecular mass (Mw) of CPTC-2 was about 73.53kDa determined by gel permeation chromatography (GPC). The structural characteristics of CPTC-2 were analyzed by gas chromatography (GC), Infrared (IR) spectrum, nuclear magnetic resonance (NMR) spectroscopy, periodate oxidation and Smith degradation studies, as well as methylation analysis. The results showed that CPTC-2 consisted of glucose, mannose, xylose, arabinose, rhamnose, and galactose with a molar ratio of 1.00:0.32:0.27:3.34:1.22:1.84. CPTC-2 was mainly composed of one type of sugar, α-glycosidic linkage. It had a backbone composed of α-(1→3) Araf, α-(1→5) Araf and α-(1→4) Galp with branches composed of α-(1→3,5) Araf and β-(1→3,6) Manp. In vitro anti-tumor experiments with SGC-7901 cells were performed and assessed by MTS (MPEG-2 Transport Stream) method and flow cytometry. The results showed CPTC-2 could inhibit the growth of SGC-7901 cells in a concentration-dependent manner via increased apoptosis. The relationship between the structure of CPTC-2 and its anti-tumor activity indicated that the α-configuration glycosidic bond residues may be essential for the anti-tumor activity of this polysaccharide.