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Dwarf and tiller-enhancing 1 regulates growth and development by influencing boron uptake in boron limited conditions in rice

Author:
Liu, Kai, Liu, Ling-Long, Ren, Yu-Long, Wang, Zhi-Quan, Zhou, Kun-Neng, Liu, Xi, Wang, Dan, Zheng, Ming, Cheng, Zhi-Jun, Lin, Qi-Bing, Wang, Jiu-Lin, Wu, Fu-Qing, Zhang, Xin, Guo, Xiu-Ping, Wang, Chun-Ming, Zhai, Hu-Qu, Jiang, Ling, Wan, Jian-Min
Source:
Plant science 2015 v.236 pp. 18-28
ISSN:
0168-9452
Subject:
Arabidopsis, Oryza sativa, RNA interference, adults, boron, corn, crop losses, crop yield, genetically modified organisms, growth retardation, leaves, molecular cloning, mutants, mutation, plasma membrane, pollen, proteins, rice, roots, shoots, staining, starvation, tillers, vegetative growth
Abstract:
Boron (B) is essential for plant growth, and B deficiency causes severe losses in crop yield. Here we isolated and characterized a rice (Oryza sativa L.) mutant named dwarf and tiller-enhancing 1 (dte1), which exhibits defects under low-B conditions, including retarded growth, increased number of tillers and impaired pollen fertility. Map-based cloning revealed that dte1 encodes a NOD26-LIKE INTRINSIC PROTEIN orthologous to known B channel proteins AtNIP5;1 in Arabidopsis and TASSEL-LESS1 in maize. Its identity was verified by transgenic complementation and RNA-interference. Subcellular localization showed DTE1 is mainly localized in the plasma membrane. The accumulation of DTE1 transcripts both in roots and shoots significantly increased within 3h of the onset of B starvation, but decreased within 1h of B replenishment. GUS staining indicated that DTE1s are expressed abundantly in exodermal cells in roots, as well as in nodal region of adult leaves. Although the dte1 mutation apparently reduces the total B content in plants, it does not affect in vivo B concentrations under B-deficient conditions. These data provide evidence that DTE1 is critical for vegetative growth and reproductive development in rice grown under B-deficient conditions.
Agid:
5412465