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Genome-wide analysis of phylogeny, expression profile and sub-cellular localization of SKP1-Like genes in wild tomato

Zhang, YueQin, Wang, CuiPing, Lin, QingFang, Gao, FengHua, Ma, Yan, Zhang, Min, Lin, YueHui, Ma, QingHu, Hua, XueJun
Plant science 2015 v.238 pp. 105-114
Arabidopsis, F-box proteins, Solanum pimpinellifolium, acid treatment, chromosomes, cytosol, fruiting, gene expression, genes, heat stress, phylogeny, protein degradation, protein-protein interactions, salicylic acid, tomatoes, ubiquitin-protein ligase
SKP1 is a core component of SCF complex, a major type of E3 ubiquitin ligase catalyzing the last step in ubiquitin-mediated protein degradation pathway. In present study, SKP1 gene family in Solanum pimpinellifolium (SSK), a wild species of tomato, was investigated. A total of 19 SSK genes were identified through homologous search. Their chromosomal locations, gene structures, phylogeny, expression profiles, sub-cellular localizations and protein–protein interaction patterns with putative F-box proteins were analyzed in detail. The high homology and similar expression patterns among clustered SSK genes in chromosome suggested that they may have evolved from duplication events and are functionally redundant. Sub-cellular localization indicated that most of the SSK proteins are distributed in both cytosol and nucleus, except for SSK8, which is detected in cytosol only. Tissue-specific expression patterns suggested that many SSK genes may be involved in tomato fruit development. Furthermore, several SSK genes were found to be responsive to heat stress and salicylic acid treatment. Based on phylogenetic analysis, expression profiles and protein interaction property, we proposed that tomato SSK1 and SSK2 might have similar function to ASK1 and ASK2 in Arabidopsis.