PubAg

Main content area

A Panel of Protease-Responsive RNA Polymerases Respond to Biochemical Signals by Production of Defined RNA Outputs in Live Cells

Author:
Pu, Jinyue, Chronis, Ian, Ahn, Daniel, Dickinson, Bryan C.
Source:
Journal of the American Chemical Society 2015 v.137 no.51 pp. 15996-15999
ISSN:
1520-5126
Subject:
RNA, engineering, gene expression, gene regulatory networks, mammals, proteolysis
Abstract:
RNA is an attractive biomolecule for biosensing and engineering applications due to its information storage capacity and ability to drive gene expression or knockdown. However, methods to link chemical signals to the production of specific RNAs are lacking. Here, we develop protease-responsive RNA polymerases (PRs) as a strategy to encode multiple specific proteolytic events in defined sequences of RNA in live mammalian cells. This work demonstrates that RNAP-based molecular recording devices can be deployed for multimodal analyses of biochemical activities or to trigger gene circuits using measured signaling events.
Agid:
5416911