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Analysis of 3-MCPD- and 3-MCPD dipalmitate-induced proteomic changes in rat liver

Author:
Braeuning, Albert, Sawada, Stefanie, Oberemm, Axel, Lampen, Alfonso
Source:
Food and chemical toxicology 2015 v.86 pp. 374-384
ISSN:
0278-6915
Subject:
acceptable daily intake, alpha-chlorohydrin, body weight, chronic exposure, fatty acid esters, foods, gastrointestinal system, glycolysis, heat treatment, kidneys, liver, oxidative stress, proteins, proteomics, rats, testes, toxicity, toxicology
Abstract:
3-Monochloropropane-1,2-diol (3-MCPD) and 3-MCPD fatty acid esters are process contaminants in foodstuff which are generated during thermal treatment. Long-term exposure to 3-MCPD or 3-MCPD esters causes toxicity especially in kidney and testis. 3-MCPD esters are efficiently hydrolyzed in the gastrointestinal tract, suggesting that their toxicity is mediated by free 3-MCPD. Combined exposure to free 3-MCPD and 3-MCPD released from 3-MCPD esters might lead to dietary consumption above the tolerable daily intake of 2 μg/kg body weight/day. Suspected mechanisms of 3-MCPD toxicity include the inhibition of glycolysis and oxidative stress. Here, a comparative proteomic approach was followed to analyze the effects of 3-MCPD or 3-MCPD dipalmitate in livers from rats exposed to 10 mg/kg body weight 3-MCPD, an equimolar dose of 3-MCPD dipalmitate, or a 4-fold lower dose of the ester during a 28-day repeated-dose feeding study. Early cellular changes were monitored in the absence of overt toxicity. A comprehensive view of 3-MCPD- or 3-MCPD dipalmitate-triggered proteomic changes in rat liver links to previously proposed mechanisms of toxicity and substantially extends our knowledge on molecular hepatic effects of 3-MCPD. Organ-independent marker proteins altered upon 3-MCPD exposure, for example DJ-1/PARK7, were identified by comparison of the proteomic patterns of kidney, testis and liver.
Agid:
5419821