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The role of structural CH compared with phenolic OH sites on the antioxidant activity of oleuropein and its derivatives as a great non-flavonoid family of the olive components: A DFT study

Author:
Hassanzadeh, Keyumars, Akhtari, Keivan, Hassanzadeh, Halaleh, Zarei, Seyed Amir, Fakhraei, Nahid, Hassanzadeh, Katayoun
Source:
Food chemistry 2014 v.164 pp. 251-258
ISSN:
0308-8146
Subject:
Olea europaea, active sites, antioxidant activity, benzene, enthalpy, free radical scavengers, gases, leaves, models, nitric oxide, oils, oleuropein, olives, phenolic compounds, solvents
Abstract:
Oleuropein and its derivatives are the main phenolic compounds of Olea europaea L. leaf and fruit. The structure–antioxidant activity relationship was considered for studying the radical scavenging activity of this non-flavonoid family of the olive components using density functional theory (DFT). The structure of these compounds were optimized employing the B3LYP/6-31G (d,p) and the role of some structural CH positions was compared with phenolic OH sites on radical scavenging. As a result, a radical unique position (C3) in the oleuropein, characterized by low BDE (Bond Dissociation Enthalpy), reasonable spin density and electron distribution, was identified. The experimental results of the previous publications of oleuropein for NO and OH scavenging confirmed the presence of this unique active site in its molecular structure. According to the results, 2,2-diphenylpicrylhydrazyl (DPPH) cannot find this non-marginal active site. Therefore, DPPH may not be a determinant assay for all antioxidant comparisons. Solvent effects were considered in all calculations using a Polarized Continuum Model (PCM) at the B3LYP/6-31G (d,p) level. Solvation calculations were carried out for benzene (ε=2.3) to simulate the oil environment compared to gas phase.
Agid:
5422676