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Antioxidant and cytoprotective properties of partridgeberry polyphenols

Author:
Bhullar, Khushwant S., Rupasinghe, H.P. Vasantha
Source:
Food chemistry 2015 v.168 pp. 595-605
ISSN:
0308-8146
Subject:
Vaccinium vitis-idaea, acetone, anthocyanins, antioxidants, cell death, chromatography, flavanols, flavonols, fractionation, isolation techniques, oxidative stress, polyphenols, reactive oxygen species, solvents, Newfoundland and Labrador
Abstract:
Partridgeberry (Vaccinium vitis-idaea) is a polyphenol-rich berry of the Ericaceous family, grown in Newfoundland and Labrador province of Canada. The aims of this study were to identify extraction solvents for the maximum recovery of polyphenols, to establish fractionation technique for isolation of major sub-classes of polyphenols, and to evaluate antioxidant and cytoprotective properties of the partridgeberry polyphenol preparations. The acidified 70% acetone was identified as the ideal solvent for the maximum recovery of polyphenols from partridgeberry. Further, aqueous two-phase extraction, column chromatography and UPLC–MS/MS were employed to produce three partridgeberry polyphenol fractions, rich in either, anthocyanins, flavan-3-ols or flavonols. All the three PPF were potent antioxidants and displayed cytoprotective activity through the activation of nuclear factor erythroid 2-related factor 2 pathway, scavenging of reactive oxygen species, and inhibition of cellular death. The current study suggests that partridgeberry has numerous potential health implications in both prevention and amelioration of various diseases involving oxidative stress.
Agid:
5423036