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A cotton-fiber-associated cyclin-dependent kinase A gene: Characterization and chromosomal location

Gao, Weifan, Saha, Sukumar, Ma, Din-Pow, Guo, Yufang, Jenkins, Johnie N., Stelly, David M.
International journal of plant genomics 2012 v.2012 pp. -
Northern blotting, active sites, adenosine triphosphate, aneuploidy, chromosome mapping, complementary DNA, cyclin-dependent kinase, flowers, gene expression, gene expression regulation, genes, hybrids, leaves, lint cotton, nucleotide sequences, protein synthesis, proteins, roots, single nucleotide polymorphism
A cotton fiber cDNA and its genomic sequences encoding an A-type cyclin-dependent kinase (GhCDKA) were cloned and characterized. The encoded GhCDKA protein contains the conserved cyclin-binding, ATP binding, and catalytic domains. Northern blot and RT-PCR analysis revealed that the GhCDKA transcript was high in 5–10 DPA fibers, moderate in 15 and 20 DPA fibers and roots, and low in flowers and leaves. GhCDKA protein levels in fibers increased from 5–15 DPA, peaked at 15 DPA, and decreased from 15 t0 20 DPA. The differential expression of GhCDKA suggested that the gene might play an important role in fiber development. The GhCDKA sequence data was used to develop single nucleotide polymorphism (SNP) markers specific for the CDKA gene in cotton. A primer specific to one of the SNPs was used to locate the CDKA gene to chromosome 16 by deletion analysis using a series of hypoaneuploid interspecific hybrids.