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Comparative study of the bioconversion process using R-(+)- and S-(–)-limonene as substrates for Fusarium oxysporum 152B

Molina, Gustavo, Bution, Murillo L., Bicas, Juliano L., Dolder, Mary Anne Heidi, Pastore, Gláucia M.
Food chemistry 2015 v.174 pp. 606-613
Fusarium oxysporum, alpha-terpineol, anaerobic conditions, biocatalysts, biotransformation, cell viability
This study compared the bioconversion process of S-(–)-limonene into limonene-1,2-diol with the already established biotransformation of R-(+)-limonene into α-terpineol using the same biocatalyst in both processes, Fusarium oxysporum 152B. The bioconversion of the S-(−)-isomer was tested on cell permeabilisation under anaerobic conditions and using a biphasic system. When submitted to permeabilisation trials, this biocatalyst has shown a relatively high resistance; still, no production of limonene-1,2-diol and a loss of activity of the biocatalyst were observed after intense cell treatment, indicating a complete loss of cell viability. Furthermore, the results showed that this process can be characterised as an aerobic system that was catalysed by limonene-1,2-epoxide hydrolase, had an intracellular nature and was cofactor-dependent because the final product was not detected by an anaerobic process. Finally, this is the first report to characterise the bioconversion of R-(+)- and S-(–)-limonene by cellular detoxification using ultra-structural analysis.