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A simplified and accurate detection of the genetically modified wheat MON71800 with one calibrator plasmid

Kim, Jae-Hwan, Park, Saet-Byul, Roh, Hyo-Jeong, Park, Sunghoon, Shin, Min-Ki, Moon, Gui Im, Hong, Jin-Hwan, Kim, Hae-Yeong
Food chemistry 2015 v.176 pp. 1-6
acetyl-CoA carboxylase, detection limit, developed countries, genes, markets, plasmids, quantitative analysis, quantitative polymerase chain reaction, statistical analysis, transgenic plants, wheat
With the increasing number of genetically modified (GM) events, unauthorized GMO releases into the food market have increased dramatically, and many countries have developed detection tools for them. This study described the qualitative and quantitative detection methods of unauthorized the GM wheat MON71800 with a reference plasmid (pGEM-M71800). The wheat acetyl-CoA carboxylase (acc) gene was used as the endogenous gene. The plasmid pGEM-M71800, which contains both the acc gene and the event-specific target MON71800, was constructed as a positive control for the qualitative and quantitative analyses. The limit of detection in the qualitative PCR assay was approximately 10 copies. In the quantitative PCR assay, the standard deviation and relative standard deviation repeatability values ranged from 0.06 to 0.25 and from 0.23% to 1.12%, respectively. This study supplies a powerful and very simple but accurate detection strategy for unauthorized GM wheat MON71800 that utilizes a single calibrator plasmid.