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Identification of phenolic compounds in red wine extract samples and zebrafish embryos by HPLC-ESI-LTQ-Orbitrap-MS
- Vallverdú-Queralt, Anna, Boix, Nuria, Piqué, Ester, Gómez-Catalan, Jesús, Medina-Remon, Alexander, Sasot, Gemma, Mercader-Martí, Mercè, Llobet, Juan M., Lamuela-Raventos, Rosa M.
- Food chemistry 2015 v.181 pp. 146-151
- Danio rerio, anthocyanins, high performance liquid chromatography, mass spectrometry, medicine, metabolites, models, pharmacology, polyphenols, red wines, toxicology
- The zebrafish embryo is a highly interesting biological model with applications in different scientific fields, such as biomedicine, pharmacology and toxicology. In this study, we used liquid chromatography/electrospray ionisation-linear ion trap quadrupole-Orbitrap-mass spectrometry (HPLC/ESI-LTQ-Orbitrap-MS) to identify the polyphenol compounds in a red wine extract and zebrafish embryos. Phenolic compounds and anthocyanin metabolites were determined in zebrafish embryos previously exposed to the red wine extract. Compounds were identified by injection in a high-resolution system (LTQ-Orbitrap) using accurate mass measurements in MS, MS2 and MS3 modes. To our knowledge, this research constitutes the first comprehensive identification of phenolic compounds in zebrafish by HPLC coupled to high-resolution mass spectrometry.