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Characterization of extended-spectrum beta-lactamases-producing Salmonella strains isolated from retail foods in Shaanxi and Henan Province, China

Author:
Yang, Baowei, Wang, Qianning, Cui, Shenghui, Wang, Yin, Shi, Chao, Xia, Xiaodong, Xi, Meili, Wang, Xin, Shi, Xianming, Wang, Dapeng, Zhang, Zengfeng, Meng, Jianghong
Source:
Food microbiology 2014 v.42 pp. 14-18
ISSN:
0740-0020
Subject:
Escherichia coli, Salmonella enterica, antibiotic resistance, beta-lactamase, ceftriaxone, foods, genes, genetic variation, nucleotide sequences, plasmids, pulsed-field gel electrophoresis, sequence analysis, serotypes, China, Indiana
Abstract:
Extended-spectrum beta-lactamases (ESBL)-producing Salmonella enterica have been reported worldwide. However, research on foodborne ESBL-producing Salmonella has been rarely conducted. One hundred and thirty eight ceftriaxone or/and cefoperazone-resistant Salmonella strains recovered from retail foods in Shaanxi and Henan Province, China, were screened for ESBL. The ESBL-producing strains were further characterized for antimicrobial resistance, pulse field gel electrophoresis (PFGE) profiles, and the presence of blaTEM, blaSHV, blaOXA, blaCTX-M, and blaPSE. The transferability of ESBL encoding genes to a susceptible Escherichia coli strain was also investigated. Thirty (21.7%) isolates were identified as ESBL positive and belonged to S. enterica serovars Indiana, Shubra, Typhimurium, and Enteritidis. S. Indiana and S. Shubra isolates were firstly identified in ESBL-producing strains. Great genetic diversity was seen among these ESBL-producing strains. Nucleotide sequence analysis revealed that blaTEM-1B was the only ESBL-encoding gene among the genes tested and was detected in 26 of 30 strains and was carried in the conjugative plasmids. The blaTEM-1B gene was transferable through conjugation at rates ranging from 4.71 × 10−7 to 7.55 × 10−6 transconjugant per recipient cell. This study provides the evidence of foodborne ESBL-producing Salmonella, and the transferability of plasmid harboring ESBL-encoding genes could possibly contribute to the dissemination of ESBL.
Agid:
5426938