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A comparison between SHF and SSSF processes from cardoon for ethanol production
- Cotana, Franco, Cavalaglio, Gianluca, Gelosia, Mattia, Coccia, Valentina, Petrozzi, Alessandro, Ingles, David, Pompili, Enrico
- Industrial crops and products 2015 v.69 pp. 424-432
- Cynara cardunculus, Saccharomyces cerevisiae, cardoons, cellulose, endo-1,4-beta-glucanase, enzymatic hydrolysis, ethanol, ethanol production, feedstocks, fermentation, hemicellulose, hydrolysis, lignin, lignocellulose, raw materials, saccharification
- The purpose of the present work is the evaluation of Cynara cardunculus L. potential in terms of ethanol production from lignocellulosic feedstock for industrial purposes. After a steam explosion (SE) pretreatment at different severity conditions, to obtain the dissolution of hemicellulose and the separation between cellulose and lignin, two different processes were tested: separate hydrolysis and fermentation (SHF) and semi-simultaneous saccharification and fermentation (SSSF). The enzymatic hydrolysis was carried out with ™Ctec2 cellulase enzyme and fermentation with Saccharomyces cerevisiae yeast.The results showed slightly better performances by SSSF process compared to SHF, especially at low severity conditions, with a maximum overall ethanol yield of 13.64g of ethanol/100g of raw material. For the SHF process the best overall ethanol yield achieved was 13.17g of ethanol/100g of raw materials. In addition, the SSSF process required at least 24h less than the SHF to achieve the same final ethanol concentration for every severity condition tested.