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Effects of glycosylation on antigenicity and immunogenicity of classical swine fever virus envelope proteins

Author:
Gavrilov, Boris K., Rogers, Kara, Fernandez-Sainz, Ignacio J., Holinka, Lauren G., Borca, Manuel V., Risatti, Guillermo R.
Source:
Virology 2011 v.420 no.2 pp. 135
ISSN:
0042-6822
Subject:
Classical swine fever virus, antibodies, antibody formation, glycoproteins, glycosylation, mutants, mutation, neutralization, post-translational modification, swine, viral proteins, viruses
Abstract:
Classical swine fever virus (CSFV) harbors three envelope glycoproteins (Eʳⁿˢ, E1 and E2). Previous studies have demonstrated that removal of specific glycosylation sites within these proteins yielded attenuated and immunogenic CSFV mutants. Here we analyzed the effects of lack of glycosylation of baculovirus-expressed Eʳⁿˢ, E1, and E2 proteins on immunogenicity. Interestingly, Eʳⁿˢ, E1, and E2 proteins lacking proper post-translational modifications, most noticeable lack of glycosylation, failed to induce a detectable virus neutralizing antibody (NA) response and protection against CSFV. Similarly, no NA or protection was observed in pigs immunized with E1 glycoprotein. Analysis of Eʳⁿˢ and E2 proteins with single site glycosylation mutations revealed that detectable antibody responses, but not protection against lethal CSFV challenge is affected by removal of specific glycosylation sites. In addition, it was observed that single administration of purified Eʳⁿˢ glycoprotein induced an effective protection against CSFV infection.
Agid:
54311
Handle:
10113/54311