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Involvement of the yciW gene in l-cysteine and l-methionine metabolism in Escherichia coli
- Kawano, Yusuke, Ohtsu, Iwao, Tamakoshi, Ai, Shiroyama, Maeka, Tsuruoka, Ai, Saiki, Kyohei, Takumi, Kazuhiro, Nonaka, Gen, Nakanishi, Tsuyoshi, Hishiki, Takako, Suematsu, Makoto, Takagi, Hiroshi
- Journal of bioscience and bioengineering 2015 v.119 no.3 pp. 310-313
- Escherichia coli, biosynthesis, cysteine, derivatization, gene overexpression, regulon, sulfur
- We here analyzed a sulfur index of Escherichia coli using LC-MS/MS combined with thiol-specific derivatization by monobromobimane. The obtained sulfur index was then applied to evaluate the l-cysteine producer. E. coli cells overexpressing the yciW gene, a novel Cys regulon, accumulated l-homocysteine, suggesting that YciW is involved in l-methionine biosynthesis.