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Cloning, expression and characterization of a novel GH5 exo/endoglucanase of Thermobifida halotolerans YIM 90462T by genome mining

Zhang, Feng, Zhang, Xiao-Mei, Yin, Yi-Rui, Li, Wen-Jun
Journal of bioscience and bioengineering 2015 v.120 no.6 pp. 644-649
Nocardiopsis alba, Nocardiopsis dassonvillei, active sites, amino acid sequences, amino acids, cellulose, endo-1,4-beta-glucanase, enzymatic hydrolysis, genes, genome mining, metal ions, pH
The 1389-bp thcel5A gene, which encodes a family 5 of glycoside hydrolases (GH5), was screened from the draft genome of Thermobifida halotolerans YIM 90462ᵀ. ThCel5A was most similar (77% identity) to a GH5 endoglucanase from Thermobifida fusca YX, followed by cellulases from Nocardiopsis dassonvillei subsp. dassonvillei DSM 43111, Nocardiopsis alba ATCC BAA-2165, and Kribbella flavida DSM 17836. The deduced amino acid sequence of ThCel5A, which consisted of 462 amino acid residues, encompassed a family 2 cellulose-binding module and a GH5 catalytic domain. Notably, ThCel5A hydrolysed soluble as well as insoluble cellulose substrates. The enzymatic hydrolysis assay showed that the activity of recombinant ThCel5A was optimized at pH 8.0 and 50°C. Moreover, it retained hydrolytic activity in the presence of various metal ions and >90% activity within the range of pH 8.0–9.0 after 30 min at 50°C. These results suggested that this enzyme has considerable potential in industrial applications.