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Anti-inflammatory activity of lupine (Lupinus angustifolius L.) protein hydrolysates in THP-1-derived macrophages

Millán-Linares, Mª del Carmen, Bermúdez, Beatriz, Yust, María del Mar, Millán, Francisco, Pedroche, Justo
Journal of functional foods 2014 v.8 pp. 224-233
CCR2 receptor, Lupinus angustifolius, anti-inflammatory activity, chemokines, chronic diseases, enzymatic hydrolysis, foods, genetic markers, humans, hydrolysates, inflammation, interleukin-1beta, interleukin-6, ligands, macrophage activation, macrophages, migratory behavior, models, nitric oxide, protein hydrolysates, quantitative polymerase chain reaction, subtilisin, tumor necrosis factors
The effect of two different lupine protein hydrolysates (LPHs) on in vitro macrophage activation in a THP-1-derived macrophage model was investigated. THP-1-derived macrophages were exposed to RPMI medium containing two LPHs obtained by enzymatic hydrolysis using two different proteases: Izyme AL and Alcalase 2.4 L. Cytokine's expression was measured by quantitative PCR. THP-1-derived macrophages exhibited attenuated expression of proinflammatory cytokines (tumor necrosis factor (TNF), IL-6, IL-1β) and increased expression of anti-inflammatory marker genes (chemokine (C-C motif) ligand 18 (CCL18)) relative to control without LPH. The anti-inflammatory effect of both hydrolysates favored M2 polarization by quenching C-C chemokine receptor type 2 (CCR2) expression and migratory capacity. Furthermore, LPHs significantly decreased nitric oxide production. Moreover, LPHs promoted the survival of human THP-1-derived macrophages. Therefore, inclusion of LPHs in foods may help to prevent chronic diseases associated with chronic inflammation.