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Anti-inflammatory activity of lupine (Lupinus angustifolius L.) protein hydrolysates in THP-1-derived macrophages
- Millán-Linares, Mª del Carmen, Bermúdez, Beatriz, Yust, María del Mar, Millán, Francisco, Pedroche, Justo
- Journal of functional foods 2014 v.8 pp. 224-233
- CCR2 receptor, Lupinus angustifolius, anti-inflammatory activity, chemokines, chronic diseases, enzymatic hydrolysis, foods, genetic markers, humans, hydrolysates, inflammation, interleukin-1beta, interleukin-6, ligands, macrophage activation, macrophages, migratory behavior, models, nitric oxide, protein hydrolysates, quantitative polymerase chain reaction, subtilisin, tumor necrosis factors
- The effect of two different lupine protein hydrolysates (LPHs) on in vitro macrophage activation in a THP-1-derived macrophage model was investigated. THP-1-derived macrophages were exposed to RPMI medium containing two LPHs obtained by enzymatic hydrolysis using two different proteases: Izyme AL and Alcalase 2.4 L. Cytokine's expression was measured by quantitative PCR. THP-1-derived macrophages exhibited attenuated expression of proinflammatory cytokines (tumor necrosis factor (TNF), IL-6, IL-1β) and increased expression of anti-inflammatory marker genes (chemokine (C-C motif) ligand 18 (CCL18)) relative to control without LPH. The anti-inflammatory effect of both hydrolysates favored M2 polarization by quenching C-C chemokine receptor type 2 (CCR2) expression and migratory capacity. Furthermore, LPHs significantly decreased nitric oxide production. Moreover, LPHs promoted the survival of human THP-1-derived macrophages. Therefore, inclusion of LPHs in foods may help to prevent chronic diseases associated with chronic inflammation.