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Anti-osteoporotic activity of sialoglycoproteins isolated from the eggs of Carassius auratus by promoting osteogenesis and increasing OPG/RANKL ratio

Xia, Guanghua, Wang, Shanshan, He, Min, Zhou, Xiaochun, Zhao, Yanlei, Wang, Jingfeng, Xue, Changhu
Journal of functional foods 2015 v.15 pp. 137-150
Carassius auratus, alkaline phosphatase, blood serum, bone density, bone formation, bone morphogenetic proteins, bone resorption, cell differentiation, collagen, eggs, ligands, mineralization, osteocalcin, osteoporosis, ovariectomy, rats, tibia, transcription factor NF-kappa B
The anti-osteoporotic activities of a sialoglycoprotein isolated from the eggs of Carassius auratus (Ca-SGP). Ca-SGP was composed of 14.33% protein, 62.81% hexose, and 19.72% N-acetylneuraminic acid (Neu5Ac). In vitro experimental results showed that Ca-SGP significantly increased MC3T3-E1 pre-osteoblastic cells' proliferation activities, promoted cell differentiation and mineralization as evidenced by increasing the contents or activities of major markers, i.e. collagen type I (COL I), osteocalcin (OCN), alkaline phosphatase (ALP) and bone nodules. Ca-SGP significantly augmented protein expressions and secretions of bone morphogenetic protein 2 (BMP-2) and osteoprotegerin (OPG), meanwhile, decreased that of receptor activator of nuclear factor κB ligand (RANKL). In vivo experimental data further revealed that Ca-SGP significantly reduced bone turnover rates, increased serum OPG/RANKL ratio and enhanced tibia bone mineral density (BMD) in the model rats of ovariectomized (OVX)-induced osteoporosis. These results suggest that Ca-SGP could improve osteoporosis by promoting osteogenesis and inhibiting bone resorption via increasing OPG/RANKL ratio, which indicates that Ca-SGP is a potential candidate functional factor for osteoporosis prevention.