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Cloning and RNA interference analysis of the salivary protein C002 gene in Schizaphis graminum

ZHANG, Yong, FAN, Jia, SUN, Jing-rui, CHEN, Ju-lian
Journal of integrative agriculture 2015 v.14 no.4 pp. 698-705
RNA interference, Schizaphis graminum, amino acids, artificial diets, complementary DNA, genes, instars, rapid amplification of cDNA ends, salivary glands, signal peptide, wheat
The full-length cDNA of functionally-unknown salivary protein C002 in Schizaphis graminum was cloned using rapid amplification of cDNA ends (RACE) and designated as SgC002 (GenBank accession no. KC977563). It is 767 bp long and encodes a protein of 190 amino acid residues with a predicted mass of 21.5 kDa and a predicted cleavage site of N-terminal signal peptide between the 24th and the 25th residues. SgC002 is specifically expressed in salivary gland with the highest level at the 2nd instar. Introducing SgC002-specific 476-siRNA, but not 546-siRNA to aphids through artificial diet significantly suppressed SgC002 expression. Silencing SgC002 gene led to lethality of the aphid on wheat plants, but not on pure artificial diet. Our study demonstrated that artificial diet-mediated RNAi can be a useful tool for research on the roles of genes in aphid salivary gland, and also provided new insights into the characteristics of C002 in wheat aphids.