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A sensitive and selective detection method for thiol compounds using novel fluorescence probe
- Zheng, Li-Qing, Li, Ying, Yu, Xiao-Dong, Xu, Jing-Juan, Chen, Hong-Yuan
- Analytica chimica acta 2014 v.850 pp. 71-77
- analytical chemistry, cysteine, detection limit, disulfide bonds, dithiothreitol, energy transfer, fluorescence, fluorescent dyes, glutathione, thiols
- In this work, a sensitive and selective detection method based on fluorescence resonance energy transfer (FRET) was developed for analyzing thiol compounds by using a novel fluorescent probe. The new fluorescent probe contains a disulfide bond which selectively reacts with nucleophilic thiolate through the thiol-disulfide exchange reaction. An obvious fluorescence recovery can be observed upon addition of the thiol compound in the fluorescent probe solution due to the thiol-disulfide exchange reaction and the destruction of FRET. This novel probe was successfully used to determine dithiothreitol (DTT), glutathione (GSH) and cysteine (Cys). The limits of detection (LOD) were 2.0μM for DTT, 0.6μM for GSH, and 0.8μM for Cys. This new detection method was further investigated in the analysis of compound amino acid injection.