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Comparative Analysis of Bacterial Diversity in Freshwater Sediment of a Shallow Eutrophic Lake by Molecular and Improved Cultivation-Based Techniques
- Tamaki, Hideyuki, Sekiguchi, Yuji, Hanada, Satoshi, Nakamura, Kazunori, Nomura, Nakao, Matsumura, Masatoshi, Kamagata, Yoichi
- Applied and environmental microbiology 2005 v.71 no.4 pp. 2162-2169
- agar, freshwater, gellan gum, lakes, ribosomal RNA, sediments
- Comparative analysis of bacterial diversity in freshwater sediment collected from a shallow eutrophic lake was performed by using 16S rRNA gene clone library and improved cultivation-based techniques. Our study demonstrated that the use of gellan gum as a gelling reagent instead of agar was more effective at increasing culturability, cultivating a diverse array of novel microbes, and reducing the gaps of the results between molecular and cultivation-based analyses.