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Different effects of myotoxins bothropstoxin-I and II from Bothrops snake venom on cation transport ATPases from murine fast twitch skeletal muscle
- Ayres, Rodrigo, Feijó, Priscilla R.O., Cintra, Adelia C.O., Tomaz, Marcelo A., Melo, Paulo A., Cunha, Valéria M.N., Quintas, Luis Eduardo M.
- Toxicon 2015 v.103 pp. 80-84
- Bothrops, Ca2-transporting ATPase, adenosinetriphosphatase, antivenoms, cations, inhibitory concentration 50, mice, models, protein synthesis, skeletal muscle, snakes, venoms
- Bothrops jararacussu venom drastically decreases sarcoplasmic Ca2+-ATPase (SERCA) protein expression in vivo and inhibits its activity in vitro, in contrast to a slight increase of Na+/K+-ATPase expression in murine EDL. We investigated the effect of myotoxins bothropstoxin-I and/or -II (BthTX-I, BthTX-II and BthTX-I+II) on this model. No changes were seen in SERCA1, SERCA2 and Na+/K+-ATPase α1 protein expression as well as 2+Ca-ATPase activity, but BthTX-II (1 μg/g) reduced Na+/K+-ATPase α2 expression by 50% one day after perimuscular injection. Interestingly, BthTX-II inhibited Ca2+-ATPase activity (IC50 around 6 nM). Our findings suggest that only BthTX-II affects ion transport ATPases, being a potent SERCA inhibitor and a putative target for antivenom drug development.