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Determining the relative sensitivity of benthic diatoms to atrazine using rapid toxicity testing: A novel method

Author:
Wood, Rebecca J., Mitrovic, Simon M., Kefford, Ben J.
Source:
The Science of the total environment 2014 v.485-486 pp. 421-427
ISSN:
0048-9697
Subject:
Achnanthidium, Amphora, Cymbella, Eunotia, Gomphonema, Navicula, algae, aquatic ecosystems, atrazine, autotrophs, benthic organisms, freshwater, herbicide resistance, risk, rocks, toxicity, toxicity testing
Abstract:
Herbicides pose a potential threat to aquatic ecosystems, especially to phototrophic organisms such as benthic diatoms. Benthic diatoms may be a valuable indicator of the toxic impacts of herbicides in aquatic systems. However, this requires information on the herbicide sensitivity of a wide range of freshwater benthic diatom taxa. Unfortunately this information is only available for a limited number of species as current methods of developing new algae toxicity tests on individual taxa are lengthy and costly. To address this issue, we developed a new rapid toxicity test method to test natural benthic communities, from which the relative herbicide sensitivity of many individual taxa can be derived. This involved the collection of natural benthic communities from rocks in situ, which were placed directly into laboratory toxicity tests. Sensitivity data for several diatom genera in a 48hour exposure toxicity test were produced, without the need for cultures or multiple site visits. After exposure to the highest treatment of atrazine (500μgL−1) there were significant declines of healthy cells in the most sensitive genera: Gomphonema declined by 74%, Amphora by 62%, Cymbella by 54% and Ulnaria by 34% compared to control levels. In contrast, the genera, Eunotia, Achnanthidium and Navicula, had no statistically significant decline in cell health. This method can identify the diatom taxa most at risk of herbicide toxicity within the natural benthic diatom community. The rapid toxicity testing method presented is a simple and effective method to obtain sensitivity data for multiple taxa within a natural benthic diatom community in a relatively short period of time.
Agid:
5455648