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Synthesis, characterization and biocompatibility of silver nanoparticles synthesized from Nigella sativa leaf extract in comparison with chemical silver nanoparticles

Author:
Amooaghaie, Rayhaneh, Saeri, Mohammad Reza, Azizi, Morteza
Source:
Ecotoxicology and environmental safety 2015 v.120 pp. 400-408
ISSN:
0147-6513
Subject:
Fourier transform infrared spectroscopy, Lolium, Nigella sativa, Vicia sativa subsp. sativa, beans, biocompatibility, canola, cell viability, crystallites, cytotoxicity, inhibitory concentration 50, leaf extracts, leaves, lettuce, mice, nanoparticles, nanosilver, phytotoxicity, raw materials, roots, scanning electron microscopes, scanning electron microscopy, seed germination, seedling growth, shoots, silver, silver nitrate, sodium, sodium citrate, stem cells, sustainable technology, wheat
Abstract:
Despite the development potential in the field of nanotechnology, there is a concern about possible effects of nanoparticles on the environment and human health. In this study, silver nanoparticles (AgNPs) were synthesized by ‘green’ and ‘chemical’ methods. In the wet-chemistry method, sodium borohydrate, sodium citrate and silver nitrate were used as raw materials. Leaf extract of Nigella sativa was used as reducing as well as capping agent to reduce silver nitrate in the green synthesis method. In addition, toxic responses of both synthesized AgNPs were monitored on bone-building stem cells of mice as well as seed germination and seedling growth of six different plants (Lolium, wheat, bean and common vetch, lettuce and canola). In both synthesis methods, the colorless reaction mixtures turned brown and UV–visible spectra confirmed the presence of silver nanoparticles. Scanning electron microscope (SEM) observations revealed the predominance of silver nanosized crystallites and fourier transform infra-red spectroscopy (FTIR) indicated the role of different functional groups in the synthetic process. MTT assay showed cell viability of bone-building stem cells of mice was further in the green AgNPs synthesized using black cumin extract than chemical AgNPs. IC50 (inhibitory concentrations) values for seed germination, root and shoot length for 6 plants in green AgNPs exposures were higher than the chemical AgNPs. These results suggest that cytotoxicity and phytotoxicity of the green synthesized AgNPs were significantly less than wet-chemistry synthesized ones. This study indicated an economical, simple and efficient ecofriendly technique using leaves of N. sativa for synthesis of AgNPs and confirmed that green AgNPs are safer than chemically-synthesized AgNPs.
Agid:
5458629