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Performance evaluation and phylogenetic characterization of anaerobic fluidized bed reactors using ground tire and pet as support materials for biohydrogen production

Barros, Aruana Rocha, Adorno, Maria Angela Tallarico, Sakamoto, Isabel Kimiko, Maintinguer, Sandra Imaculada, Varesche, Maria Bernadete Amâncio, Silva, Edson Luiz
Bioresource technology 2011 v.102 no.4 pp. 3840-3847
Clostridium, Enterobacter, bacteria, fluidized beds, glucose, hydrogen, hydrogen production, nucleotide sequences, phylogeny, polyethylene, positron-emission tomography, ribosomal RNA, sequence analysis, sludge, temperature, tires, total solids, wastewater
This study evaluated two different support materials (ground tire and polyethylene terephthalate [PET]) for biohydrogen production in an anaerobic fluidized bed reactor (AFBR) treating synthetic wastewater containing glucose (4000mgL⁻¹). The AFBR, which contained either ground tire (R1) or PET (R2) as support materials, were inoculated with thermally pretreated anaerobic sludge and operated at a temperature of 30°C. The AFBR were operated with a range of hydraulic retention times (HRT) between 1 and 8h. The reactor R1 operating with a HRT of 2h showed better performance than reactor R2, reaching a maximum hydrogen yield of 2.25molH₂mol⁻¹ glucose with 1.3mg of biomass (as the total volatile solids) attached to each gram of ground tire. Subsequent 16S rRNA gene sequencing and phylogenetic analysis of particle samples revealed that reactor R1 favored the presence of hydrogen-producing bacteria such as Clostridium, Bacillus, and Enterobacter.