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Bioaugmentation of DDT-contaminated soil by dissemination of the catabolic plasmid pDOD

Author:
Gao, Chunming, Jin, Xiangxiang, Ren, Jingbei, Fang, Hua, Yu, Yunlong
Source:
Journal of Environmental Sciences 2015 v.27 pp. 42-50
ISSN:
1001-0742
Subject:
Cellulomonas, DDT (pesticide), Escherichia coli, Sphingobacterium, bioaugmentation, plasmids, soil bacteria, soil degradation, soil types, sowing, temperature
Abstract:
A plasmid transfer-mediated bioaugmentation method for the enhancement of dichlorodiphenyltrichloroethane (DDT) degradation in soil was developed using the catabolic plasmid pDOD from Sphingobacterium sp. D-6. The pDOD plasmid could be transferred to soil bacteria, such as members of Cellulomonas, to form DDT degraders and thus accelerate DDT degradation. The transfer efficiency of pDOD was affected by the donor, temperature, moisture, and soil type. Approximately 50.7% of the DDT in the contaminated field was removed 210days after the application of Escherichia coli TG I (pDOD-gfp). The results suggested that seeding pDOD into soil is an effective bioaugmentation method for enhancing the degradation of DDT.
Agid:
5471501