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An Intron Sense Suppression Construct Targeting Soybean FAD2-1 Requires a Double-Stranded RNA-Producing Inverted Repeat T-DNA Insert
- Mroczka, Andrew, Roberts, Peter D., Fillatti, JoAnne J., Wiggins, B. Elizabeth, Ulmasov, Tim, Voelker, Toni
- Plant physiology 2010 v.153 no.2 pp. 882-891
- Glycine max, genes, loci, oleic acid, polyunsaturated fatty acids, seeds, small interfering RNA, soybeans, transfer DNA
- We demonstrate that the transformation of soybean (Glycine max) with sense suppression constructs using intron sequences from the fatty acid oleyl Δ12 desaturase gene FAD2-1A leads to efficient and specific reduction of FAD2-1 transcripts in developing seeds, increased oleic acid, and decreased polyunsaturated fatty acids. The related FAD2-2 transcripts are only marginally affected. Despite screening a large number of independent transformants, no single-copy efficacious transformants could be found. Invariably, all the least complex transgenic loci have two T-DNA copies in an inverted repeat configuration, centered at the right borders. We show that this T-DNA configuration produces an inverted repeat transcript and that small interfering RNAs accumulate against the target sequence.