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Fluorescence Polarization Immunoassay for Highly Efficient Detection of Imidaclothiz in Agricultural Samples

Ma, Ming, Chen, Mo, Feng, Lu, You, Hongjie, Yang, Rui, Boroduleva, Anna, Hua, Xiude, Eremin, Sergei A., Wang, Minghua
Food analytical methods 2016 v.9 no.9 pp. 2471-2478
antibody detection, apples, cabbage, cucumbers, detection limit, fluorescence, high performance liquid chromatography, immunoassays, inhibitory concentration 50, monoclonal antibodies, paddies, pears, rice, tomatoes, tracer techniques
A homogeneous fluorescence polarization immunoassay (FPIA) based on a monoclonal antibody for the detection of imidaclothiz was developed. Two fluorescein-labeled imidaclothiz tracers containing two different bridge lengths were synthesized and purified. Under optimal conditions, the 4-aminofluorescein-labeled imidaclothiz conjugate (AMF-labeled imidaclothiz), which contains a shorter bridge length, showed a higher sensitivity in the FPIA for detecting imidaclothiz, and the full analysis was achieved in less than 11 min. The IC₅₀ and limit of detection (LOD, IC₁₀) were 87.94 ± 10.18 and 0.57 ± 0.16 μg/L, respectively. The spiked recoveries were 83 to 117 % measured in tomato, pear, rice, apple, cucumber, cabbage, and paddy water, with RSDs of 5 to 12 %. Furthermore, the results of FPIA for the authentic samples correlated well with those acquired by HPLC. Overall, the developed FPIA provided a simple, rapid, sensitive, and accurate method that was used for the quantitative detection of imidaclothiz in agricultural samples.