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A high-density genetic linkage map of bronze loquat based on SSR and RAPD markers

Fukuda, Shinji, Ishimoto, Keiichiro, Sato, Shusei, Terakami, Shingo, Hiehata, Naofumi, Yamamoto, Toshiya
Tree genetics & genomes 2016 v.12 no.4 pp. 80
Eriobotrya japonica, Malus, Pyrus, apples, chromosome mapping, genes, genetic distance, linkage groups, loci, loquats, microsatellite repeats, pears, quantitative trait loci, random amplified polymorphic DNA technique, Taiwan
We constructed a high-density genetic linkage map of bronze loquat (Eriobotrya deflexa) by using a three-way cross of loquat (Eriobotrya japonica) × (loquat × bronze loquat) and simple sequence repeat (SSR) and random amplified polymorphic DNA (RAPD) markers. The positions of the SSR loci used in this study were previously identified on reference maps of pears (Pyrus spp.) and apples (Malus spp.). The map of bronze loquat (‘Taiwan loquat No. 1’) consisted of 308 loci including 167 SSRs (8 loquat, 57 pear, and 102 apple SSRs), 140 RAPDs, and the loquat canker resistance gene Pse-a on 19 linkage groups covering a genetic distance of 1036 cM. Almost all loquat linkage groups were aligned to the pear consensus map by using at least two pear or apple SSRs, suggesting that positions and linkages of SSR loci were well conserved between loquat and pear and between loquat and apple. The constructed map may be used to determine the location of genes and quantitative trait loci of interest and to analyze genome synteny in the tribe Pyreae, subfamily Spiraeoideae of the family Rosaceae.