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Characterization of a novel bacteriocin produced by Lactobacillus plantarum ST8SH and some aspects of its mode of action

Todorov, Svetoslav Dimitrov, Holzapfel, Wilhelm, Nero, Luis Augusto
Annals of microbiology 2016 v.66 no.3 pp. 949-962
Enterococcus, Klebsiella pneumoniae, Lactobacillus plantarum, Listeria, Streptococcus, antibacterial properties, antimicrobial peptides, bacteriocins, biopreservation, food pathogens, genetic similarity, growth retardation, humans, lactic acid bacteria, mechanism of action
Bacteriocins produced by lactic acid bacteria (LAB) are ribosomally synthesized antimicrobial peptides, with a diverse mode of bactericidal activity. This study focused on characterization of the bactericidal activity of bacteriocin ST8SH, with special attention to control of Listeria and Enteroccus species. Lactobacillus plantarum ST8SH produces a bacteriocin of the pediocin PA-1 family (sharing 96 % similarity on genetic level) with activity against several LAB, Enterococcus spp., Klebsiella pneumoniae, Listeria spp., Streptococcus spp. and some other human and foodborne pathogens. Addition of bacteriocin ST8SH to exponential or stationary phase cultures of L. monocytogenes ScottA and E. faecalis ATCC 19433 inhibited growth for 12 h. The effects of bacteriocin ST8SH on L. monocytogenes ScottA and E. faecalis ATCC 19433 were recorded indirectly based on enzyme, protein and nucleotide material leakage. Considering the antimicrobial activity of bacteriocin ST8SH against the tested microorganisms, and the physiological characteristics of Lb. plantarum ST8SH, either the bacteriocin or the strain may be used as tools for biopreservation.