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Molecular mapping of stripe rust resistance gene YrJ22 in Chinese wheat cultivar Jimai 22

Chen, Can, He, Zhonghu, Lu, Jialing, Li, Jia, Ren, Yan, Ma, Chuanxi, Xia, Xianchun
Molecular breeding 2016 v.36 no.8 pp. 118
Puccinia striiformis f. tritici, Triticum aestivum, chromosome mapping, chromosomes, cultivars, disease control, disease resistance, dominant genes, genetic distance, genetic markers, genotyping, microsatellite repeats, races, seedlings, single nucleotide polymorphism, stripe rust, wheat, China
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is an important disease of wheat worldwide. Host resistance is the best way to control the disease. Genetic analysis of F₂ and F₂:₃ populations from an Avocet S/Jimai 22 cross indicated that stripe rust resistance in Jimai 22 was conferred by a single dominant gene, tentatively designated YrJ22. A total of 377 F₂ plants and 127 F₂:₃ lines were tested with Chinese Pst race CYR32 and genotyped with simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers. A linkage map was constructed with five SSR and two SNP markers. Xwmc658 and IWA1348 flanked YrJ22 at genetic distances of 1.0 and 7.3 cM, proximally and distally, respectively. The chromosomal location was confirmed using Chinese Spring nulli-tetrasomic, ditelosomics and deletion lines. Seedling reactions to 21 Pst races demonstrated differences in specificity between YrJ22 and other resistance genes on chromosome 2AL, indicating that YrJ22 is likely to be a new wheat stripe rust resistance gene.