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RLIN1, encoding a putative coproporphyrinogen III oxidase, is involved in lesion initiation in rice
- Sun, Changhui, Liu, Linchuan, Tang, Jiuyou, Lin, Aihong, Zhang, Fantao, Fang, Jun, Zhang, Genfa, Chu, Chengcai
- Journal of genetics and genomics 2011 v.38 no.1 pp. 29-37
- biosynthesis, exons, leaves, lesions (plant), missense mutation, molecular cloning, phenotype, reactive oxygen species, recessive genes, rice, screening, stems, transfer DNA
- Lesion mimic is necrotic lesions on plant leaf or stem in the absence of pathogenic infection, and its exact biological mechanism is varied. By a large-scale screening of our T-DNA mutant population, we identified a mutant rice lesion initiation 1 (rlin1), which was controlled by a single nuclear recessive gene. Map-based cloning revealed that RLIN1 encoded a putative coproporphyrinogen III oxidase in tetrapyrrole biosynthesis pathway. Sequencing results showed that a G to T substitution occurred in the second exon of RLIN1 and led to a missense mutation from Asp to Tyr. Ectopic expression of RLIN1 could rescue rlin1 lesion mimic phenotype. Histochemical analysis demonstrated that lesion formation in rlin1 was light-dependent accompanied by reactive oxygen species accumulated. These results suggest that tetrapyrrole participates in lesion formation in rice.