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Self-paired monoclonal antibody lateral flow immunoassay strip for rapid detection of Acidovorax avenae subsp. citrulli

Zeng, Haijuan, Guo, Wenbo, Liang, Beibei, Li, Jianwu, Zhai, Xuzhao, Song, Chunmei, Zhao, Wenjun, Fan, Enguo, Liu, Qing
Analytical and bioanalytical chemistry 2016 v.408 no.22 pp. 6071-6078
Acidovorax avenae subsp. citrulli, detection limit, gold, immunoaffinity chromatography, immunoassays, leaves, microorganisms, monoclonal antibodies, polymerase chain reaction, rapid methods, stems
We screened a highly specific monoclonal antibody (McAb), named 6D, against Acidovorax avenae subsp. citrulli (Aac). Single McAb 6D was used as both nanogold-labeled antibody and test antibody to develop a single self-paired colloidal gold immunochromatographic test strip (Sa-GICS). The detection limit achieved using the Sa-GICS approach was 10⁵ CFU/mL, with a result that can be observed by the naked eye within 10 min. Moreover, Sa-GICS can detect eight strains of Aac and display no cross-reactions with other pathogenic plant microorganisms. Artificial contamination experiments demonstrated that Sa-GICS would not be affected by impurities in the leaves or stems of the plants and were consistent with the PCR results. This is the first report on the development of a colloidal gold immunoassay strip with self-paired single McAb for the rapid detection of Aac. Graphical Abstract Schematic representation of the test strip