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Development of an ELISA to detect clenbuterol in swine products using a new approach for hapten design

Bui, Quoc Anh, Vu, Thi Huynh Han, Ngo, Vo Ke Thanh, Kennedy, Ivan R., Lee, N. Alice, Allan, Robin
Analytical and bioanalytical chemistry 2016 v.408 no.22 pp. 6045-6052
beta-adrenergic agonists, clenbuterol, cross reaction, detection limit, enzyme-linked immunosorbent assay, epinephrine, growth promotion, haptens, markets, pork, surveys, swine, Vietnam
This research outlines the application of an enzyme-linked immunosorbent assay (ELISA) for the analysis of clenbuterol in animal products. Our assay showed good sensitivity for clenbuterol (0.4 ng/g or 0.4 ppb) and low detection limit (0.09 ng/g or 0.09 ppb). A low cross-reactivity for other β₂-agonist drugs such as salbutamol, terbutaline, and epinephrine led to formatting an ELISA kit considered to have a high specificity for clenbuterol. A survey of Ho Chi Minh City pork market was conducted as part of the validation of our ELISA. ELISA results showed a surprisingly high value of contamination. However, it will be necessary to conduct a more statistically valid replicated survey with evaluation by other instrumental methods to obtain a definite conclusion. This ELISA kit will be used to monitor growth promoter residues in Vietnam’s animal products.