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Development of an ELISA to detect clenbuterol in swine products using a new approach for hapten design

Author:
Bui, Quoc Anh, Vu, Thi Huynh Han, Ngo, Vo Ke Thanh, Kennedy, Ivan R., Lee, N. Alice, Allan, Robin
Source:
Analytical and bioanalytical chemistry 2016 v.408 no.22 pp. 6045-6052
ISSN:
1618-2642
Subject:
beta-adrenergic agonists, clenbuterol, cross reaction, detection limit, enzyme-linked immunosorbent assay, epinephrine, growth promotion, haptens, markets, pork, surveys, swine, Vietnam
Abstract:
This research outlines the application of an enzyme-linked immunosorbent assay (ELISA) for the analysis of clenbuterol in animal products. Our assay showed good sensitivity for clenbuterol (0.4 ng/g or 0.4 ppb) and low detection limit (0.09 ng/g or 0.09 ppb). A low cross-reactivity for other β₂-agonist drugs such as salbutamol, terbutaline, and epinephrine led to formatting an ELISA kit considered to have a high specificity for clenbuterol. A survey of Ho Chi Minh City pork market was conducted as part of the validation of our ELISA. ELISA results showed a surprisingly high value of contamination. However, it will be necessary to conduct a more statistically valid replicated survey with evaluation by other instrumental methods to obtain a definite conclusion. This ELISA kit will be used to monitor growth promoter residues in Vietnam’s animal products.
Agid:
5480820